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KMID : 0365819710110020207
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Journal of Pusan Medical College
1971 Volume.11 No. 2 p.207 ~ p.212
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The Effect of Insecticides on Rat Cholinesterase Activities
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Abstract
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This study was undertaken to observe the inhibitory effect of insecticides on the cholinesterase activities of brain, red blood cell and plasma in rats.
Rats were used as experimental animals throughout this study. Fifty five rats were divided into 11 groups;
Group 1: control, Group 2: Dipterex injected, Group 3: DDT injected, Group 4: Dipterex plus DDT injected, Group 5: EPN injected, Group 6: BHC injected, Group 7: EPN plus BHC injected, Group8: EPN plus DDT injected, Group 9: DDVP given orally, Group 10: Nicotine given orally, Group 11: DDVP plus. nicotine given orally.
Animals were sacrificed by decapitation and exsanguination. Blood was collected in centrifuge tubes containing heparin and the plasma and red cells were separated by centrifugation. Brains were removed, weighed and homogenized in Ringer solution.
Cholinesterase activities were measured manometrically according to the method of Ammon. The results of this investigaton were as follows:
1. Brain cholinesterase activities were strongly inhibited by DDVP, followed by EPN, DDVP plus nicotine, dipterex, dipterex plus DDT and EPN plus DDT in this order.
2. Red cell cholinesterase activities were remarkably inhibited by DDVP, followed by EPN, DDVP plus nicotine, EPN plus DDT and DDT in this order.
3. Plasma cholinesterase activities were significantly inhibited by DDVP, followed by EPN, nicotine, DDVP plus nicotine and dipterex plus DDT in this order.
From the result thus obtained, it may be concluded that such organo phosphate as DDVP, EPN and dipterex have inhibitory effect on the cholinesterase activities of brain, red blood cell and plasma, and that the complex administration of organo phosphate and organ chlorin does not show the additive function on the cholinesterase activity of the tissue examined.
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KEYWORD
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